Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
D112N | 121% of wild-type activity | Enterobacter sp. |
F95A | 120% of wild-type activity | Enterobacter sp. |
F95A/D112N | 395% of wild-type activity | Enterobacter sp. |
F95A/S157I | 218% of wild-type activity | Enterobacter sp. |
F95A/V151I | 333% of wild-type activity | Enterobacter sp. |
F95E | 26% of wild-type activity | Enterobacter sp. |
F95I | 148% of wild-type activity | Enterobacter sp. |
F95I/D112N | 418% of wild-type activity | Enterobacter sp. |
F95I/S157I | 299% of wild-type activity | Enterobacter sp. |
F95I/V151I | 323% of wild-type activity | Enterobacter sp. |
F95K | 18% of wild-type activity | Enterobacter sp. |
F95L | 188% of wild-type activity | Enterobacter sp. |
F95L/D112N | 485% of wild-type activity | Enterobacter sp. |
F95L/D112N/S157I | 430% of wild-type activity | Enterobacter sp. |
F95L/D112N/V151I | 1354% of wild-type activity | Enterobacter sp. |
F95L/D112N/V151I | approximately 34fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid, possibly due to formation of a compact active site compared with that of the wild-type | Enterobacter sp. |
F95L/S157I | 528% of wild-type activity | Enterobacter sp. |
F95L/V151I | 746% of wild-type activity | Enterobacter sp. |
F95L/V151I/S157I | 484% of wild-type activity | Enterobacter sp. |
F95Q | 48% of wild-type activity | Enterobacter sp. |
F95R | 19% of wild-type activity | Enterobacter sp. |
S157I | 171% of wild-type activity | Enterobacter sp. |
V151I | 174% of wild-type activity | Enterobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 62.9% residual activity | Enterobacter sp. | |
Cu2+ | 1 mM, almost complete loss of activity | Enterobacter sp. | |
Fe2+ | 1 mM, 94.3% residual activity | Enterobacter sp. | |
Fe3+ | 1 mM, 1.7% residual activity | Enterobacter sp. | |
Mg2+ | 1 mM, 69.7% residual activity | Enterobacter sp. | |
Mn2+ | 1 mM, 67.9% residual activity | Enterobacter sp. | |
Ni2+ | 1 mM, 81.8% residual activity | Enterobacter sp. | |
Zn2+ | 1 mM, almost complete loss of activity | Enterobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter sp. | C6F3U5 | - |
- |
Enterobacter sp. Px6-4 | C6F3U5 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.3 | - |
wild-type, pH 7.0, 37°C | Enterobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferulate | - |
Enterobacter sp. | 4-vinylguaiacol + CO2 | - |
? | |
ferulate | - |
Enterobacter sp. Px6-4 | 4-vinylguaiacol + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FADase | - |
Enterobacter sp. |